Custom Cell Culture Services & Primary Cell Expansion

Cell Culture Services Built for Speed, Consistency & Scientific Confidence

You handle the breakthroughs.

Let ABS Bio handle your cell culture.

From master and working cell banks to large-scale assay ready culture production, ABS Bio helps biopharma teams reduce internal burden, improve reproducibility, and keep research programs moving.

Shift your focus from cell production to discovery by letting ABS Bio provide you with the high-quality cells and tissues your research. Our US-based team streamlines and accelerates your research with a reliable supply of cells for your experiments, antibody production, and high-throughput screening efforts.

Our cell culture services premise is simple: If you need cells for your therapeutic or diagnostic research, our expert team can:

  • Culture them to your specifications
  • Harvest them per your protocol and needs
  • Store them in secure controlled environment
  • Ship them where and when you need them

Related Resources

Cell Line Scale-Up

ABS Bio supports high-volume production for demanding research programs.

Capabilities include:

  • Cell culture expansion of billions of adherent and suspension cells
  • Cell banking
    • Master Banks
    • Working Banks
    • Assay-ready Banks (up to 1000 vials per batch)
  • Hybridoma expansion
  • Conditioned media production
  • Tangential flow filtration for protein concentration
  • Cell treatment and customized processing

Why it matters:

  • Reduce internal lab burden
  • Improve batch consistency
  • Accelerate screening programs
  • Minimize experiment-to-experiment variability

Culture Types

  • Suspension Cell Culture
  • Adherent Cell Culture / Anchorage Dependent
  • Mixed Cell Culture

Cell Lines

The ABS Bio Cell Culture team has grown more than 5000 unique cell lines for clients. Typical lines include:

  • CHO (Chinese Hamster Ovary)
  • Cancer Cell Lines
    • Examples: K562 and VCap (Vertebral Cancer of the Prostate)
  • Human Cell Lines
    • Examples: HEK AND HELA
  • Animal Cells
  • Transfected Cell Lines

Vessel Types

  • Flasks
  • Cell Stacks (Multi-layer flasks)
  • Shaker Suspension
  • Roller Bottles
  • Plates

End Products

  • Cryopreserved Cells
  • Cell Pellets
  • Membrane Preps
  • Fixed Cells
  • Histoplugs (FFPE blocks)
  • Live Cells

Why Biopharma Teams Outsource Cell Culture to ABS Bio

  • Free internal scientists for higher-value work
  • Improve consistency across assays
  • Scale rapidly without hiring staff
  • Reduce contamination and QC risks
  • Access specialized expertise
  • Ensure continuity of cell supply
  • Support aggressive project timelines
  • Expertise built on 35+ years of supporting biopharma

An Extension of Your Research Team

In addition to technical cell culture expertise and robust quality control, the ABS Bio team communicates with client teams frequently to keep projects on-track and to troubleshoot issues before they become problems. We reach out in real time if a question arises, and we encourage you to do the same.

We are committed to operate as if we are an extension of your team!

Assay-Ready Cells Without the Production Burden

Ready for an experiment, but your cells aren’t?

We can grow large batches of cells according to your specifications, reducing experiment-to-experiment variability. Our cells are cryopreserved in convenient aliquots and then stored in liquid nitrogen in cryovials, microtiter plates, or your preferred format until you’re ready to use them.

ABS Bio can prepare, store, and deliver Master, Working and Assay-Ready Cell Banks for your use whenever you are ready. Our assay-ready cells reduce delays and increase reproducibility.

Cell Line Engineering

Our team can prepare stable cell lines or transiently transfect cells according to your specifications. Choose from a variety of transfection methods, or send us specific protocols. We can scale up large quantities of transiently transfected cells, and we also have protein and membrane purification capabilities.

Protein Expression Verification Methods:

We routinely produce adipocytes, epithelial cells, fibroblasts, muscle cells, neurons, pancreatic cells, tumor cells, and many other cell types. We will gladly work with you to engineer cell lines for your specific needs.

Primary Cells

Because of ABS’s access to human and animal tissues and our expertise in cell culture solutions, ABS is in a prime position to provide primary cells. We can also provide primary tumor cell cultures through our partnerships with a large number of hospitals.

Cell isolations and purification are done on a custom basis. We’ve prepared human splenocytes, cardiomyocytes, T and B cells, neutrophils, macrophages, muscle cells, fibroblasts, and many others.

Reliable Cell Banking, Storage & On-Demand Fulfillment

ABS Bio operates a CAP-accredited, ISO 9001:2015-certified biorepository to securely store and ship your cells on demand.

Cell Culture Terminology

Adherent Cells

Cells that require attachment to a surface, such as a culture flask or plate, in order to grow and proliferate.

Anchorage Dependence

The requirement of certain cells to attach to a substrate for survival and growth under in vitro conditions.

Assay-Ready Cells

Cells that have been prepared, qualified, and supplied in a format suitable for immediate experimental use.

Aseptic Technique

Procedures used to prevent microbial contamination during cell culture handling and processing.

Authentication

The process of verifying the identity and origin of a cell line, often using STR profiling or genetic analysis.

Batch Consistency

The ability to produce cell cultures with uniform characteristics and performance across multiple production runs.

Biobanking

The systematic collection, processing, storage, and management of biological materials for research or clinical use.

Cell Bank

A collection of cryopreserved cells stored for future use under controlled conditions.

Cell Culture

The growth and maintenance of cells outside their natural environment under controlled laboratory conditions.

Cell Doubling Time

The amount of time required for a cell population to double in number.

Cell Expansion

The process of increasing cell numbers through controlled growth and passaging.

Cell Line

A population of cells derived from a primary source that can be propagated in culture over multiple generations.

Cell Line Development

The process of generating, selecting, and optimizing cell lines with desired biological characteristics.

Cell Line Engineering

The modification of cells using genetic or molecular techniques to introduce or alter specific traits.

Cell Morphology

The size, shape, and structural characteristics of cells observed during culture.

Cell Passage

The transfer of cells from one culture vessel to another to maintain healthy growth conditions.

Cell Pellet

A concentrated mass of cells collected through centrifugation.

Cell Viability

A measure of the proportion of living, healthy cells within a population.

Cell-Based Assay

An experimental procedure that uses living cells to evaluate biological activity, toxicity, potency, or drug response.

Conditioned Media

Culture media that contains biological factors secreted by cells during growth.

Confluency

The percentage of a culture surface covered by adherent cells.

Contamination

The unintended introduction of microorganisms, chemicals, or cross-contaminating cells into a culture.

Cryopreservation

The preservation of cells at ultra-low temperatures to maintain viability for long-term storage.

Cryoprotectant

A substance, such as DMSO, used to protect cells from damage during freezing.

Culture Media

A nutrient-rich solution used to support cell growth and maintenance.

Differentiation

The process by which cells develop specialized structures or functions.

Dissociated Cells

Cells separated from tissue or cell aggregates into a single-cell suspension.

Expansion Culture

A phase of cell culture focused on increasing total cell yield.

FBS (Fetal Bovine Serum)

A common cell culture supplement that provides growth factors, hormones, and nutrients.

Feeder Cells

Supportive cells used to promote the growth or maintenance of another cell type.

Frozen Cell Stock

Cryopreserved vials of cells maintained for future experimental or production use.

Good Cell Culture Practice (GCCP)

Recommended standards and procedures designed to ensure quality and reproducibility in cell culture operations.

Hybridoma

A fused cell line used to produce monoclonal antibodies.

Incubator

A controlled environment chamber used to maintain optimal temperature, humidity, and COâ‚‚ levels for cell growth.

In Vitro

Experimental work performed outside a living organism, typically in a laboratory setting.

Mammalian Cell Culture

The cultivation of mammalian cells under controlled laboratory conditions.

Master Cell Bank (MCB)

A well-characterized collection of original cell aliquots used as the primary source for future cell production.

Mycoplasma Testing

Testing performed to detect contamination by mycoplasma organisms, which can compromise cell culture integrity.

Passage Number

The number of times a cell culture has been subcultured or transferred.

Primary Cells

Cells isolated directly from living tissue that retain many characteristics of the original tissue.

Quality Control (QC)

Procedures used to verify that cells meet defined specifications for identity, purity, viability, and performance.

Recombinant Protein Expression

The production of proteins by genetically engineered cells.

Scale-Up

The process of increasing culture volume or production capacity to generate larger quantities of cells or biological products.

Serum-Free Media

Culture media formulated without animal serum components.

Single-Cell Suspension

A preparation in which individual cells are uniformly dispersed in liquid media.

Suspension Cells

Cells capable of growing freely suspended in liquid culture media.

Tangential Flow Filtration (TFF)

A filtration technique used to concentrate or purify cells, proteins, or biological materials.

Thaw Recovery

The restoration of viable cell growth following cryopreservation and thawing.

Transfection

The introduction of nucleic acids into cells to alter gene expression or cellular behavior.

Viable Cell Count

The number of living cells present in a sample, typically measured using staining or automated counting methods.

Working Cell Bank (WCB)

A secondary bank of cells derived from the Master Cell Bank and used for routine production or research activities.