Cell Line Engineering & Development Services for Drug Discovery

ABS Bio provides cell line engineering services to support research, drug discovery, and therapeutic development programs. Our team can generate stable cell lines using client-supplied protocols or a variety of established engineering approaches. Working in collaboration with our flow cytometry specialists, we can generate engineered cell pools with defined expression profiles or isolate individual clones based on project requirements.

We also support transient transfection projects and can scale production of both stable and transient cell lines. Each project is supported by a dedicated team member to help ensure clear communication, efficient project management, and reliable execution from initiation through final delivery.

A successful cell line engineering project starts with high-quality materials. ABS Bio can support project initiation through plasmid scale-up and DNA preparation for transfection workflows, protein purification for electroporation studies, and virus generation for transduction projects.

ABS Bio utilizes multiple cell engineering approaches, including transfection, viral transduction, and electroporation. Following engineering, cell populations can be enriched through sorting to isolate desired pools or individual clones.

Our team can isolate single cells and single-cell-derived colonies using methods aligned with client protocols. Approaches include limiting dilution and cell sorting, enabling generation of cell populations tailored to specific research objectives.

Following pool or clone generation, ABS Bio verifies expression of the protein of interest using a range of analytical techniques, including Western analysis, ELISA, immunohistochemistry (IHC), and flow cytometry.

Once expression has been confirmed and selected clones or pools have been identified, ABS Bio can expand and bank cell lines for future research use.